The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.
经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上
位复性后，ELISA结果证实其具有良好
结合β3抗原活性。
The expression product of ScFv was purified through Ni-NTA agarose metal affinity resin column and renatured.
经金属螯合亲和层析柱(Ni-NTA agarose)纯化和凝胶柱上位复性后，ELISA结果证实其具有良好结合β3抗原活性。
Tigogenin was separated completely from the crude sapogenins by macroporous resin column chromatography,only simple solvent Ilelute was used in the column of the separate process.
用大孔树脂为吸附
法分离过程中仅需要简单溶-Ⅱ,分离出单一组分剑麻皂甙元占粗皂甙元比率高达70%以上。
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